Journal: Cell reports

Article Title: Species-specific MARCO-alphavirus interactions dictate chikungunya virus viremia

doi: 10.1016/j.celrep.2023.112418

Figure Lengend Snippet:

Article Snippet: Surface expression of MARCO and mMxra8 was assessed by flow cytometry with monoclonal antibody ED31 (4 μg/mL; Bio-Rad MCA1849; RRID: AB_2140591), polyclonal human MARCO antibody (1 μg/mL; R&D AF7586), or monoclonal murine Mxra8 antibody (4 μg /mL; a gift from M. Diamond, Washington University), followed by secondary antibodies: phycoerythrin (PE)-anti-rat IgG1 antibody (BioLegend 407407; RRID: AB_2565987), fluorescein isothiocyanate (FITC)-anti-goat IgG antibody (Jackson ImmunoResearch 705–096-147; RRID: AB_2340402), and FITC anti-Armenian hamster IgG antibody (BioLegend 405502; RRID: AB_315020), respectively.

Techniques: Control, Virus, Recombinant, Mutagenesis, Transfection, Gene Expression, Clone Assay, Plasmid Preparation, Software, Flow Cytometry

Journal: PLoS ONE

Article Title: CD36 Differently Regulates Macrophage Responses to Smooth and Rough Lipopolysaccharide

doi: 10.1371/journal.pone.0153558

Figure Lengend Snippet: (A) S-LPS at 1 μg/ml enhances binding of rCD36 to plate-adsorbed rCD14. (B) CD36-/- PEMs exhibit neither basal nor 1 μg/ml S-LPS-stimulated rCD14 binding. (C) S-LPS-stimulated binding of rCD14 to PEMs is blocked by anti-CD36 mAb, but not by anti-TLR2 or anti-TLR4/MD-2 mAb. (D) During 40 min co-incubation at 37°C, 200 ng/ml S-LPS stimulates much stronger rCD14 binding to PEMs than the same concentration of R-LPS. Graphs show means ± SEM of 3–4 replicates, obtained in single experiments, each representative of at least 3 similar experiments performed. Data on graphs C and D were analyzed with ANOVA, followed by the Dunnett’s post-test. *, p < 0.05; HRP-anti-Fc, HRP-conjugated F(ab’) 2 fragments of goat antibodies specific for Fc fragments of human IgG.

Article Snippet: Bound rCD36 was detected by 1-h incubation with 2 μg/ml of polyclonal goat anti-mouse CD36 Ab (R&D Systems), followed by 1-h incubation with 4 μg/ml of HRP-conjugated F(ab’) 2 fragments of donkey anti-goat IgG Ab (Rockland) in BSA-PBS.

Techniques: Binding Assay, Incubation, Concentration Assay